Mgr. David Doležel, Ph.D.

Funkce: Vedoucí laboratoře - Laboratoř molekulární chronobiologie

Kontaktní údaje

Telefon: 420387775239
E-mail: dolezel@entu.cas.cz

Popis

MAIN RESEARCH INTERESTS

· Insect seasonality – diapause; architecture of the photoperiodic timer (at molecular, genetic and cellular levels), geographic variability of the photoperiodic timer, Juvenile hormone signaling in insect reproduction.

· Pyrrhocoris apterus as a new insect model – endocrinology (neuropeptides, evolution of neuropeptide receptors), reproductive behavior, circadian clock, phylogeography of P. apterus and its adaptation, technique development (RNA interference, genome editing, organ cultures).

· Drosophila melanogaster – temperature compensation of the circadian clock, CRISPR/CAS9 and related tools for targeted mutagenesis.

· Evolution of circadian genes – mostly evolution of clock geens in insects and Metazoa, magnetoreception (using insect models), circadian clock in protozoa (small hobby project on Chromera velia, organism related to Plasmodium). For details, check current research projects on the web page of the Laboratory of Molecular Chronobiology

MAJOR COLLABORATIONS

Christian Wegener, University of Wuerzburg, Germany; Project: Neuropeptides in P. apterus
Fernando Noriega, Florida Int. University, USA; Project: Juvenile hormone in P. apterus
Robert Hanus, Institute of Organic Chemistry and Biochemistry, CAS, Czech Republic; Project: Juvenile hormone and beyond

Mentor of PhD students

Current:

Markéta Hejníková
Enrico Bullo (co-supervisor: Dr. Smykal)
Magdalena Kaniewska (co-supervisor: Dr. Kotwica-Rolinska)
Nirav Thakkar

Past: ·

Samarjeet Singh (defended in 2020)
Lenka Pivarčiová (Chodáková) (defended in 2019) ·
Olga Bazalová (defended in 2016)
Adam Bajgar, PhD (defended in June 2013)

Mentor of undergraduate students

Current:

Adéla Hejzlarová (co-supervisor Nirav Thakkar)
Václav Brabec (co-supervisor Nirav Thakkar)

Past:

Maly Bertolutti (co-supervisor Nirav Thakkar) · Simona Fišerová · František Kitzberger
Marion Sieber (MSc – 2014),
Katka Švehlová (MSc – 2013)
Olga Pecková (MSc – 2012)
Martina Jonáková (MSc – 2012)
Silvie Fexová (MSc – 2010)
Olga Bazalová (MSc – 2010)
Juraj Dobrovodský (BSc - 2012 )
Olga Pecková (BSc 2010)
Eva Stehliková (BSc 2010)

Odborný životopis

Curriculum vitae

BORN: 1974; Czech Republic

CURRENT POSITION(S)

group leader, Institute of Entomology, Biology Center CAS, Czech R. (full appointment)

lecturer, Faculty of Science, University of South Bohemia (current appointment 10%)

EDUCATION AND RESEARCH EXPERIENCE

1994-1997 B.Sc. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

1997 three-month-stay, The University of Arizona (Tucson), David Galbraith´s lab., USA.

1997-1999 M.Sc. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

1999-2003 Ph.D. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

2004-2007 Postdoctoral Fellow, Brandeis U. (Laboratory of J. C. Hall), Waltham, MA, USA.

2007-2010 Postdoctoral Fellow, Institute of Entomology, Biology Center ASCR, Č. Budějovice, CZ

2011-2013 Researcher, Institute of Entomology, Biology Center ASCR, Č. Budějovice, CZ

Since 2013 Group leader, Laboratory of Chronobiology, Biology Center ASCR, Č. Budějovice, CZ

MEMBERSHIP IN SCIENTIFIC SOCIETIES

· The Society on Research on Biological Rhythms, · network member, Marie Curie ITN “INsecTIME” http://insectime.org/main/ (FP7)

· International Society for Evolutionary Protistology – 1997-2001

OTHER PROFESSIONAL ACTIVITIES

Reviewer for journals (on regular basis): Insect Biochemistry and Molecular Biology, Proceedings of the National Academy of Sciences USA, Journal of Biological Rhythms, Journal of Insect Physiology, PLoS ONE, Entomological Science

Referee of a Ph.D. thesis: (University of Jyvaskyla, Finland; University of Leicester, UK; Charles University, Prague; Valencia, Spain)

PUBLICATIONS

39 peer-reviewed papers (including 1x invited review), >800 citations, h-index 19 (Scopus), 19 (WOS)

Publikace

Celkem nalezeno: 47 záznamů
Hejníková M., Nouzová M., Ramirez C.E., Fernandez-Lima F., Noriega F., Doležel D. (2022) Sexual dimorphism of diapause regulation in the hemipteran bug Pyrrhocoris apterus Insect Biochemistry and Molecular Biology 142 : 103721. DOI: 10.1016/j.ibmb.2022.103721
Kotwica-Rolinska J., Chodáková L., Smýkal V., Damulewicz M., Provazník J., Wu C., Hejníková M., Chvalová D., Doležel D. (2022) Loss of Timeless Underlies an Evolutionary Transition within the Circadian Clock. Molecular Biology and Evolution 39 : msab346. DOI: 10.1093/molbev/msab346
Kotwica-Rolinska J., Damulewicz M., Chodáková L., Krištofová L., Doležel D. (2022) Pigment dispersing factor is a circadian clock output and regulates photoperiodic response in the linden bug, Pyrrhocoris apterus. Frontiers in Physiology 13 : article number: 884909. DOI: 10.3389/fphys.2022.884909
Kozelková T., Doležel D., Grunclová L., Kučera M., Perner J., Kopáček P. (2021) Functional characterization of the insulin signaling pathway in the hard tick Ixodes ricinus Ticks and Tick-borne Diseases 12 : 101694. DOI: 10.1016/j.ttbdis.2021.101694
Netušil R., Tomanová K., Chodáková L., Chvalová D., Doležel D., Ritz T., Vacha M. (2021) Cryptochrome-dependent magnetoreception in a heteropteran insect continues even after 24 h in darkness. Journal of Experimental Biology 224 : article number: jeb243000. DOI: 10.1242/jeb.243000
Kaniewska M., Vaněčková H., Doležel D., Kotwica-Rolinska J. (2020) Light and temperature synchronizes locomotor activity in the linden bug, Pyrrhocoris apterus. Frontiers in Physiology 11 : article number: 242. DOI: 10.3389/fphys.2020.00242
Kotwica-Rolinska J., Krištofová L., Chvalová D., Pauchová L., Provazník J., Hejníková M., Sehadová H., Lichý M., Vaněčková H., Doležel D. (2020) Functional analysis and localisation of a thyrotropin-releasing hormone-type neuropeptide (EFLa) in hemipteran insects. Insect Biochemistry and Molecular Biology 122 : article number: 103376. DOI: 10.1016/j.ibmb.2020.103376
Smýkal V., Pivarči M., Provazník J., Bazalová O., Jedlička P., Lukšan O., Horák A., Vaněčková H., Beneš V., Fiala I., Hanus R., Doležel D. (2020) Complex evolution of insect insulin receptors and homologous decoy receptors, and functional significance of their multiplicity. Molecular Biology and Evolution 37 : 1775-1789. DOI: 10.1093/molbev/msaa048
Bartoš P., Netušil R., Slabý P., Doležel D., Ritz T., Vácha M. (2019) Weak radiofrequency fields affect the insect circadian clock. Journal of the Royal Society Interface 16 : article number: 20190285. DOI: 10.1098/rsif.2019.0285
Doležel D. (2019) Chronobiology: The circadian clock under extreme photoperiods. Current Biology 29 : R1176-R1178. DOI: 10.1016/j.cub.2019.09.061
Kotwica-Rolinska J., Chodáková L., Chvalová D., Krištofová L., Fenclová I., Provazník J., Wu C., Bertolutti M., Doležel D. (2019) CRISPR/Cas9 genome editing introduction and optimization in the non-model insect Pyrrhocoris apterus. Frontiers in Physiology 10 : article 891. DOI: 10.3389/fphys.2019.00891
Paolucci S., Dalla Benetta E., Salis L., Doležel D., van de Zande L., Beukeboom L.W. (2019) Latitudinal variation in circadian rhythmicity in Nasonia vitripennis. Behavioral Sciences 9 : article number: 115. DOI: 10.3390/bs9110115
Singh S., Giesecke A., Damulewicz M., Fexová S., Mazzotta G.M., Stanewsky R., Doležel D. (2019) New Drosophila circadian clock mutants affecting temperature compensation induced by targeted mutagenesis of timeless. Frontiers in Physiology 10 : article number: 1442. DOI: 10.3389/fphys.2019.01442
Ditrich T., Janda V., Vaněčková H., Doležel D. (2018) Climatic variation of supercooling point in the linden bug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). Insects 9 : article number 144. DOI: 10.3390/insects9040144
Bazalová O., Doležel D. (2017) Daily Activity of the Housefly, Musca domestica, Is Influenced by Temperature Independent of 3' UTR period Gene Splicing G3: Genes, Genomes, Genetics 7 : 2637-2649. DOI: 10.1534/g3.117.042374
Ibrahim E. A. S., Hejníková M., Shaik H. A., Doležel D., Kodrík D. (2017) Adipokinetic hormone activities in insect body infected by entomopathogenic nematode. J. Insect Physiol. 98 : 347-355. DOI: 10.1016/j.jinsphys.2017.02.009
Kotwica-Rolinska J., Pivarčiová L., Vaněčková H., Doležel D. (2017) The role of circadian clock genes in the photoperiodic timer of the linden bug, Pyrrhocoris apterus, during the nymphal stage. Physiological Entomology 42 : 266-273. DOI: 10.1111/phen.12197
Bazalová O., Kvicalova M., Valkova T., Slaby P., Bartos P., Netusil R., Tomanova K., Braeunig P., Lee H., Šauman I., Damulewicz M., Provazník J., Pokorny R., Doležel D., Vacha M. (2016) Cryptochrome 2 mediates directional magnetoreception in cockroaches. Proceedings of the National Academy of Sciences of the United States of America 113 : 1660-1665. DOI: 10.1073/pnas.1518622113
Pivarčiová L., Vaněčková H., Provazník J., Wu C., Pivarči M., Pecková O., Bazalová O., Čada Š., Kment P., Kotwica-Rolinska J., Doležel D. (2016) Unexpected geographic variability of the free running period in the linden bug, Pyrrhocoris apterus. Journal of Biological Rhythms 31 : 568-576. DOI: 10.1177/0748730416671213
Urbanová V., Bazalová O., Vaněčková H., Doležel D. (2016) Photoperiod regulates growth of male accessory glands through juvenile hormone signaling in the linden bug, Pyrrhocoris apterus. Insect Biochemistry and Molecular Biology 70 : 184-190.

Více publikací