Ing. Petr Hušek, DrSc.

Job Position: Research Scientist

Publications
Total found: 50 records
Šimek P., Hušek P., Zahradníčková H. (2019) Heptafluorobutyl chloroformate-based sample preparation protocol for nonchiral and chiral amino acid analysis by gas chromatography-mass spectrometry. In: Alterman M. A. (ed.): Amino Acid Analysis: Methods and Protocols, Methods in Molecular Biology, Vol. 2030, Springer Science+Business Media, LLC, part of Springer Nature 2019, 455 pp., ISBN 978-1-4939-9638-4. 237-251.
DOI: 10.1007/978-1-4939-9639-1_18
Hušek P., Švagera Z., Hanzlíková D., Karlínová I., Řimnáčová L., Zahradníčková H., Šimek P. (2018) GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol. In: Theodoridis G. A., Gika H. G., Wilson I. D. (eds): Metabolomic Profiling: Methods and Protocols , Methods in Molecular Biology, Vol. 1738, Humana Press, New York pp. 159-181.
DOI: 10.1007/978-1-4939-7643-0_11
Moos M., Šimek P., Petrus K., Hušek P. (2017) Poloprovoz MetaboAuto® - PGL MXP-S kit. POLOPROVOZ
Hušek P., Švagera Z., Hanzlíková D., Zahradníčková H., Opekarová I., Šimek P. (2016) Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry. Journal of Chromatography A 1443 : 211-232.
Moos M., Šimek P., Petrus K., Hušek P. (2016) Poloprovoz MetaboAuto® - PGL HFBCF MXP-U. POLOPROVOZ
Hušek P., Šimek P. (2014) A new method for immediate derivatization of hydroxyl groups by fluoroalkyl chloroformates and its application for the determination of sterols and tocopherols in human serum and amniotic fluid by gas chromatography-mass spectrometry. Journal of Chromatography A 1339 : 154-167.
DOI: 10.1016/j.chroma.2014.03.007
Moos M., Hušek P., Šimek P. (2014) Příprava metabolomické analytické metody s manuální přípravou vzorků a její přizpůsobení plně automatizovanému procesu. POLOPROVOZ
Řimnáčová L., Hušek P., Šimek P. (2014) A new method for immediate derivatization of hydroxyl groups by fluoroalkyl chloroformates and its application for the determination of sterols and tocopherols in human serum and amniotic fluid by gas chromatography-mass spectrometry. Journal of Chromatography A 1339 : 154-167.
DOI: 10.1016/j.chroma.2014.03.007
Šimek P., Hušek P., Švagera Z., Hanzlíková D., Zahradníčková H. (2013) In situ heptafluorobutyl chloroformate mediated derivatization – liquid liquid extraction for GC-MS urine metabolomics. In: Program from ExTech 2013, 15th International Symposium on Advances in Extraction Technologies, August 5-7, 2013, Joao Pessoa, Brazil.
Šimek P., Zahradníčková H., Cimlová J., Hořicová P., Berková P., Košťál V., Šimáčková K., Fesl J., Hušek P. (2013) Non-chiral and chiral profiling strategies for simultaneous GC-MS & LC-MS analysis of endogenous primary & secondary metabolites. Book of Abstracts from 8th International Conference on Instrumental Methods of Analysis: Modern Trends and Application, September 15-19, 2013, Thessaloniki, Greece 67.
Cimlová J., Hořicová P., Švagera Z., Hušek P., Šimek P. (2012) In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry J. Mass. Spectrom. 47 : 294-302.
DOI: 10.1002/jms.2952
Hušek P. (2012) Letter to the Editor regarding “GC–MS with ethyl chloroformate derivatization for comprehensive analysis of metabolites in serum and its application to human uremia". Anal. Bioanal. Chem. 403 : 2785-2786.
DOI: 10.1007/s00216-011-5615-x
Hušek P., Švagera Z., Hanzlíková D., Šimek P. (2012) Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography. J. Pharm. Biomed. Anal. 67- 68 : 159-162.
DOI: 10.1016/j.jpba.2012.04.027
Šimek P., Hušek P., Zahradníčková H. (2012) Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acids analysis by gas chromatography In: Alterman M. A., Hunziker P. (eds): Amino Acid Analysis: Methods and Protocols, Springer Science & Bussiness Media, New York. ISBN 978-1-61779-444-5 137-152.
DOI: 10.1007/978-1-61779-445-2_13
Šimek P., Zahradníčková H., Cimlová J., Makuderová L., Hušek P. (2012) Alkyl chloroformate-mediated derivatization – extraction platform for in-situ metabolite labeling and parallel nonchiral and chiral GC-MS & LC-MS analysis In: Book of Abstracts and Program from Advances in Chromatography and Electrophoresis & Chiranal 2012, June 11-14, 2012, Olomouc, pp. 46-47. (Acta Universitatis Palackianae Olomucensis)
Švagera Z., Hanzlíková D., Šimek P., Hušek P. (2012) Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography–mass spectrometry Anal. Bioanal. Chem. 402 : 2953-2963.
DOI: 10.1007/s00216-012-5727-y
Řimnáčová L., Šimek P., Cimlová J., Zahradníčková H., Hušek P. (2011) POSTER: Alkyl chloroformates labelling strategies and their application in the identification and analysis of protic metabolites and xenometabolites. In: IMA 2011, Chania, Řecko, 18. – 22.9.2011
Šimek P., Cimlová J., Zahradníčková H., Hušek P. (2011) Alkyl chloroformate labeling strategies and their application in the identification and analysis of protic metabolites and xenometabolites. In: Abstracts from The 7th International Conference on Instrumental Methods of Analysis Modern Trends and Applications, September 18-22, 2011, Chania Crete, Greece, p. 254 + poster.
Cimlová J., Zahradníčková H., Makuderová L., Berková P., Hušek P., Šimek P. (2010) Parallel Profiling of Small Molecules Metabolites in Urine after in Situ Treatment with Ethyl Chloroformate 7th Aegean Analytical Chemistry Days AACD 2010, P-3-42, abstrakt s. 240, 28.9.-3.10.Lesvos 2010, Řecko 2010 : 240.
Hušek P., Zahradníčková H., Šimek P., Švagera Z. (2010) In-situ pretreatment of urine with fluoroakryl chloroformates as a novel GC-MS metabolite profiling strategy In: Abstracts from Metabolomics 2010, June 27-July 1, 2010, Amsterdam, The Netherlands poster PP2-008, p. 190..

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CONTACT

Biology Centre CAS
Institute of Entomology
Branišovská 1160/31
370 05 České Budějovice

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