Mgr. Hana Sehadová, Ph.D.

Job Position: Head of the unit - Bioscience Imaging and Histology Unit , Research Scientist - Laboratory of Molecular Chronobiology

Contact details

Phone: 420387775273
E-mail: sehadova@yahoo.com

Curriculum vitae

Education and Research Experience

  • 2008-present Researcher, Department of Molecular Biology and Genetics, Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Ceske Budejovice
  • 2006-2008 Postdoctoral Research Assistant, School of Biological and Chemical Sciences, Queen Mary University, London, United Kingdom
  • 2004-2006 The Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship For Foreign Researchers, Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Japan
  • 2003-2004 Research Associate, Faculty of Agriculture, Kobe University, Kobe, Japan
  • 1997-2003 Research Assistant, Institute of Entomology, Academy of Sciences of the Czech Republic, Ceske Budejovice
  • 1997-2002 Ph.D. student, (Molecular, Cell Biology and Genetics), Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic
  • 1995-1997 M.Sc. student, (Cell and Developmental Biology), Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic
  • 1995-1997 Pedagogy and Psychology, Faculty of Pedagogy, University of South Bohemia, Ceske Budejovice, Czech Republic
  • 1992-1995 B.Sc. student, (General Biology) Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic
  • 1988-1992 Secondary school, Pelhrimov, Czech Republic

 

Diploma Projects

  • 1997-2002 Doctor diploma research project: "Mechanisms of the Circadian Rhythm Regulation in the American Cockroach Periplaneta americana." (Supervisor: RNDr. Ivo Sauman, Ph.D.)
  • 1995-1997 Master diploma research project: "Distribution of lin 19 Protein in Drosophila melanogaster " (Supervisor: RNDr. Ivo Sauman, Ph.D.)
  • 1992-1995 Bachelor diploma research project: "Chromosomal Localization of lin 19 Gene in Drosophila melanogaster" (Supervisor: Dr. Maria A. Filippova)

 

Popularization of Science

  • Weyda F., Dammer J., Sehadová H., Havelka A., Kolínová M., Pflegerová J. (2014) Vybrané metody studia hmyzích fosilií v jantaru. Živa 2014/5: 198-202
  • Sehadová H., Nebesářová J., Kaňa R. (2015) Mikroskopy v rukou biologů. Vesmír 2015/6: 348-351

 

Training and Professional Development

  • Confocal Microscope Training - Zeiss LSM510-META (Queen Mary University, London, United Kingdom - September 2006)
  • Confocal Microscope Training - Olympus FluoView FV1000 (Czech Academy of Sciences, Ceske Budejovice, Czech Republic - May 2008)
  • Imaris Program Training for 3D and 4D Real-Time Interactive Image Visualization (Czech Academy of Sciences, Ceske Budejovice, Czech Republic - May 2008)
  • Demonstration and One Month Training on Luminescence Microscope Olympus LV200 (Queen Mary University, London, United Kingdom - June 2008)

 

Skills

  • immunocystochemistry (paraplast sections, cryosections, wholemonths)
  • in situ hybridization
  • fluorescence and confocal microscopy
  • Westernblot analysis
  • Northernblot analysis
  • molecular cloning (PCR, sequence analyses)
  • real-time measurements of gene expression (RT-PCR; Topcount Luminometer, Perkin Elmer; Luminoview LV200, Olympus)
  • analysis of Drosophila locomotor activity on “Drosophila Activity Monitoring System” and related computer analyses in MATLAB program
  • computer skills (Microsoft Office, Adobe Acrobat, Adobe Photoshop, Adobe Illustrator, Corel Draw, Microsoft PowerPoint, and programs for confocal image analysis: Imaris, ImageJ, LSM Image Examiner) 
  • laboratory management associated with setting up the laboratory

 

Teaching Experience

  • 1997-2002 Teaching Assistant, Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic. Courses: Developmental Biology, Methods in Cell Biology, Methods in Molecular Biology
  • 2003-2006 Co-supervision of Ph.D. Diploma Projects, Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Japan
  • September 2003 Elitza Petkova Markova “Structures and functional roles of transcription regulators in circadian system of Bombyx mori ”
  • February 2006 Qi-Miao Shao “Molecular mechanism of circadian clock in two ground crickets: Dianemobius nigrofasciatus and Allonemobius allardi [Orthoptera: Gryllidae]” 
  • August 2006 Le Thi Dieu Trang “Structures and functional roles of casein kinases and N-acetyltransferases in the regulation of circadian oscillation in Bombyx mori"
  • 2007-2008 Supervision of Project Student, School of Biological and Chemical Sciences, Queen Mary University, London, United Kingdom
  • May 2008 Joyce Wambui Kibaru “Searching for new genes that are involved in the circadian clock of the fruitfly, Drosophila melanogaster"
  • May 2009 Yasamin Tahir 
  • May 2009 Anthony Edet 
  • 2010 - present Lecturer in education projects of the Biology Centre, Academy of Sciences of the Czech Republic, Ceske Budejovice and courses of the Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic

 

Projects

  • EKOTECH” – Multidisciplinary education of experts for the use of biotechnologies in the ecology-related fields. Course: Modern methods in circadian biology
  • VĚDRO” – Science for the Public / Path to Sustainable Development. Course: Modern methods in circadian biology

 

Lectures

  • Methods of Cell Study (guarantor František Weyda)
  • Advanced Methods in Molecular Biology (guarantor David Doležel)
  • Developmental Biology (guarantor František Sehnal, Jan Zrzavý)

Publications
Total found: 32 records
Kludkiewicz B., Kučerová L., Koníková T., Strnad H., Hradilová M., Žaloudíková A., Sehadová H., Koník P., Sehnal F., Žurovec M. (2019) The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella. Insect Biochemistry and Molecular Biology 106 : 28-38.
DOI: 10.1016/j.ibmb.2018.11.003
Koubová J., Jehlík T., Kodrík D., Sábová M., Šíma P., Sehadová H., Závodská R., Čapková Frydrychová R. (2019) Telomerase activity is upregulated in the fat bodies of pre-diapause bumblebee queens (Bombus terrestris). Insect Biochemistry and Molecular Biology 115 : 103241.
DOI: 10.1016/j.ibmb.2019.103241
Shaik H. A., Shaik A., Sehadová H., Kodrík D. (2019) Responses of sericotropin to toxic and pathogenic challenges: possible role in defense of the wax moth Galleria mellonella. Comparative Biochemistry and Physiology C (accepted).
Tomková Š., Sehadová H., Žurovec M. (2019) Imunohistochemická lokalizace adipokinetického hormonu (AKH) a jeho receptoru (AKHR) v centrální nervové soustavě larvy a dospělce bource morušového (Bombyx mori). Sborník abstraktů z konference 95. Fyziologické dny - konference české a slovenské fyziologické společnosti, 5.-7. února 2019, 3. Lékařská fakulta UK, Praha, pp. 53.
Zabelina V., Vrchotová M., Sehadová H., Sehnal F. (2019) Ranný embryonální vývoj bource morušového. Sborník abstraktů z konference 95. Fyziologické dny - konference české a slovenské fyziologické společnosti, 5.-7. února 2019, 3. Lékařská fakulta UK, Praha, pp. 53.
Bačák D., Sehadová H., Oros M. (2018) CLSM observation of plerocercoid morphology: interspecific differences of two species of Dibothriocephalus (Cestoda). Sborník abstraktů z konference 24th Helminthological Days 2018 – Czech Society for Parasitology, 7.-11. May 2018, Faculty of Science, Charles University, Praha, pp8., ISBN 978-80-7444-057-1
Sehadová H., Schöttner K., Závodská R., Košťál V. (2016) Funkce cirkadiánních hodin v larvách octomilky (Chymomyza costata). Sborník abstraktů z konference 92. Fyziologické dny - konference české a slovenské fyziologické společnosti, 2.-4. února 2016, Přírodovědecká fakulta JU, České Budějovice, pp. 87, ISBN 978-80-7394-563-3
Žurovec M., Yonemura N., Kludkiewicz B., Sehnal F., Kodrík D., Marques Cota Vieira L. M., Kučerová L., Strnad H., Koník P., Sehadová H. (2016) Sericin Composition in the Silk of Antheraea yamamai. Biomacromolecules DOI: 10.1021/acs.biomac.6b00189
Gladysz M., Guzik J., Sehadová H., Baran B., Bembenek J., Dolezych B. (2015) Immunochemical detection of the crustacean cardioactive peptide in the cephalic ganglia cockroaches (Blattodea: Blattidae). European Journal of Entomology 112 : 235-244.
DOI: 10.14411/eje.2015.035
Schöttner K., Sehadová H., Závodská R., Košťál V. (2015) The neuroanatomy and function of the circadian clock in larvae of the fruit fly Chymomyza costata. XIV European Biological Rhythms Society (EBRS) and IV World Congress of Chronobiology (WCC), 2 - 6 August 2015, The University of Manchester, Manchester, UK, P104.
Sehadová H., Nebesářová J., Kaňa R. (2015) Mikroskopy v rukou biologů Vesmír 6 : 348-351.
Lowe N., Rees J., Roote J., Ryder E., Armean I., Johnson G., Drummond E., Lilley K., Russell S., Johnston D., Azevedo R., Sehadová H. (2014) Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library. Development 141 : 3994-4005.
DOI: 10.1242/dev.111054
Weyda F., Dammer J., Sehadová H., Havelka A., Kolínová M., Pflegerová J. (2014) Vybrané metody studia hmyzích fosilií v jantaru. Živa 62 : číslo 5: 198-202.
Gentile C., Sehadová H., Simoni A., Chen C., Stanewsky R. (2013) Cryptochrome antagonizes synchronization of Drosophila’s circadian clock to temperature cycles. Current Biology 23 : 185-195.
DOI: 10.1016/j.cub.2012.12.023
Szular J., Sehadová H., Gentile C., Szabo G., Chou W., Britt S., Stanewsky R. (2012) Rhodpsin 5- and Rhodopsin 6-mediated clock synchronization in Drosophila melanogaster in independent of retinal phospholipase C-beta signaling Journal of Biological Rhythms 27 : 25-36.
DOI: 10.1177/0748730411431673
Chen K., Peschel N., Závodská R., Sehadová H., Stanewsky R. (2011) QUASIMODO, a Novel GPI-Anchored Zona Pellucida Protein Involved in Light Input to the Drosophila Circadian Clock. Current Biology 21 : 719-729.
DOI: 10.1016/j.cub.2011.03.049
Chen K., Peschel N., Závodská R., Sehadová H., Stanewsky R. (2010) Functional study of a novel gene, quasimodo, in the Drosophila circadian clock (meeting abstract). Journal of Neurogenetics 24 : 20.
Glaser F., Sehadová H., Giesecke A., Gentile C., Simoni A., Stanewsky R. (2009) Temperature entrainment mechanisms in Drosophila involve the novel gene nocte and peripheral organ to brain signaling (meeting abstract). Journal of Neurogenetics 23 : 50.
Sehadová H., Glazer F., Gentile C., Simoni A., Giesecke A., Albert J., Stanewsky R. (2009) Temperature entrainment of Drosophila’s circadian clock involves the gene nocte and signaling from peripheral sensory tissues to the brain. Neuron 64 : 251-266.
Yoshii T., Wülbeck C., Sehadová H., Veleri S., Bichler D., Stanewsky R., Helfrich-Förster C. (2009) The neuropeptide pigment-dispersing factor adjusts period and phase of Drosophila's clock. Journal of Neuroscience 29 : 597-610.

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CONTACT

Biology Centre CAS
Institute of Entomology
Branišovská 1160/31
370 05 České Budějovice

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