Mgr. David Doležel, Ph.D.

Job Position: Head of Laboratory - Laboratory of Molecular Chronobiology

Contact details

Phone: 420387775239



· Insect seasonality – diapause; architecture of the photoperiodic timer (at molecular, genetic and cellular levels), geographic variability of the photoperiodic timer, Juvenile hormone signaling in insect reproduction.

· Pyrrhocoris apterus as a new insect model – endocrinology (neuropeptides, evolution of neuropeptide receptors), reproductive behavior, circadian clock, phylogeography of P. apterus and its adaptation, technique development (RNA interference, genome editing, organ cultures).

· Drosophila melanogaster – temperature compensation of the circadian clock, CRISPR/CAS9 and related tools for targeted mutagenesis.

· Evolution of circadian genes – mostly evolution of clock geens in insects and Metazoa, magnetoreception (using insect models), circadian clock in protozoa (small hobby project on Chromera velia, organism related to Plasmodium). For details, check current research projects on the web page of the Laboratory of Molecular Chronobiology



  •  Christian Wegener, University of Wuerzburg, Germany; Project: Neuropeptides in P. apterus
  •  Fernando Noriega, Florida Int. University, USA; Project: Juvenile hormone in P. apterus
  •  Robert Hanus, Institute of Organic Chemistry and Biochemistry, CAS, Czech Republic; Project: Juvenile hormone and beyond


Mentor of PhD students


  • Markéta Hejníková
  • Enrico Bullo (co-supervisor: Dr. Smykal)
  • Magdalena Kaniewska (co-supervisor: Dr. Kotwica-Rolinska)
  • Nirav Thakkar

Past: ·

  • Samarjeet Singh (defended in 2020)
  • Lenka Pivarčiová (Chodáková) (defended in 2019) ·
  • Olga Bazalová (defended in 2016)
  •  Adam Bajgar, PhD (defended in June 2013)


Mentor of undergraduate students


  • Adéla Hejzlarová (co-supervisor Nirav Thakkar)
  • Václav Brabec (co-supervisor Nirav Thakkar)


  •  Maly Bertolutti (co-supervisor Nirav Thakkar) · Simona Fišerová · František Kitzberger
  •  Marion Sieber (MSc – 2014),
  •  Katka Švehlová (MSc – 2013)
  •  Olga Pecková (MSc – 2012)
  •  Martina Jonáková (MSc – 2012)
  •  Silvie Fexová (MSc – 2010)
  •  Olga Bazalová (MSc – 2010)
  •  Juraj Dobrovodský (BSc - 2012 )
  •  Olga Pecková (BSc 2010)
  •  Eva Stehliková (BSc 2010)
Curriculum vitae

Curriculum vitae

BORN: 1974; Czech Republic


group leader, Institute of Entomology, Biology Center CAS, Czech R. (full appointment)

lecturer, Faculty of Science, University of South Bohemia (current appointment 10%)



1994-1997 B.Sc. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

1997 three-month-stay, The University of Arizona (Tucson), David Galbraith´s lab., USA.

1997-1999 M.Sc. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

1999-2003 Ph.D. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

2004-2007 Postdoctoral Fellow, Brandeis U. (Laboratory of J. C. Hall), Waltham, MA, USA.

2007-2010 Postdoctoral Fellow, Institute of Entomology, Biology Center ASCR, Č. Budějovice, CZ

2011-2013 Researcher, Institute of Entomology, Biology Center ASCR, Č. Budějovice, CZ

Since 2013 Group leader, Laboratory of Chronobiology, Biology Center ASCR, Č. Budějovice, CZ



· The Society on Research on Biological Rhythms, · network member, Marie Curie ITN “INsecTIME” (FP7)

· International Society for Evolutionary Protistology – 1997-2001



Reviewer for journals (on regular basis): Insect Biochemistry and Molecular Biology, Proceedings of the National Academy of Sciences USA, Journal of Biological Rhythms, Journal of Insect Physiology, PLoS ONE, Entomological Science

Referee of a Ph.D. thesis: (University of Jyvaskyla, Finland; University of Leicester, UK; Charles University, Prague; Valencia, Spain)



39 peer-reviewed papers (including 1x invited review), >800 citations, h-index 19 (Scopus), 19 (WOS)

Total found: 56 records
Doležel D. (2024) Sociosexual interactions: A clock synchronized by smell. Current Biology 34 : R284-R286.
DOI: 10.1016/j.cub.2024.02.065
Tůmová Š., Doležel D., Jindra M. (2024) Conserved and unique roles of bHLH-PAS transcription factors in insects – From clock to hormone reception Journal of Molecular Biology 436 : article number: 168332.
DOI: 10.1016/j.jmb.2023.168332
Doležel D. (2023) Molecular Mechanism of the Circadian Clock. In: Numata H, Tomioka K (ed) Insect Chronobiology, 1st edn. Springer, Singapore; ISBN 978-981-99-0725-0. 49-84.
DOI: 10.1007/978-981-99-0726-7_4
Kaniewska M., Chvalová D., Doležel D. (2023) Impact of photoperiod and functional clock on male diapause in cryptochrome and pdf mutants in the linden bug Pyrrhocoris apterus. Journal of Comparative Physiology A Early View : DOI: 10.1007/s00359-023-01647-5
Richtová J., Bazalová O., Horák A., Tomčala A., Gonepogu V., Oborník M., Doležel D. (2023) Circadian rhythms and circadian clock gene homologs of complex alga Chromera velia Frontiers in Plant Science 14 : 1226027.
DOI: 10.3389/fpls.2023.1226027
Smýkal V., Chodáková L., Hejníková M., Briediková K., Wu C., Vaněčková H., Chen P., Janovská A., Kyjaková P., Vácha M., Doležel D. (2023) Steroid receptor coactivator TAIMAN is a new modulator of insect circadian clock. PLoS Genetics 19 : article number: e1010924.
DOI: 10.1371/journal.pgen.1010924
Smýkal V., Doležel D. (2023) Evolution of proteins involved in the final steps of juvenile hormone synthesis. Journal of Insect Physiology 145 : article number: 104487.
DOI: 10.1016/j.jinsphys.2023.104487
Thakkar N., Hejzlarová A., Brabec V., Doležel D. (2023) Germline Editing of Drosophila Using CRISPR-Cas9-based Cytosine and Adenine Base Editors. The CRISPR Journal 6 : 557-569.
DOI: 10.1089/crispr.2023.0026
Hejníková M., Nouzová M., Ramirez C.E., Fernandez-Lima F., Noriega F., Doležel D. (2022) Sexual dimorphism of diapause regulation in the hemipteran bug Pyrrhocoris apterus Insect Biochemistry and Molecular Biology 142 : 103721.
DOI: 10.1016/j.ibmb.2022.103721
Kotwica-Rolinska J., Chodáková L., Smýkal V., Damulewicz M., Provazník J., Wu C., Hejníková M., Chvalová D., Doležel D. (2022) Loss of Timeless Underlies an Evolutionary Transition within the Circadian Clock. Molecular Biology and Evolution 39 : article number: msab346.
DOI: 10.1093/molbev/msab346
Kotwica-Rolinska J., Damulewicz M., Chodáková L., Krištofová L., Doležel D. (2022) Pigment dispersing factor is a circadian clock output and regulates photoperiodic response in the linden bug, Pyrrhocoris apterus. Frontiers in Physiology 13 : article number: 884909.
DOI: 10.3389/fphys.2022.884909
Thakkar N., Giesecke A., Bazalová O., Martinek J., Smýkal V., Stanewsky R., Doležel D. (2022) Evolution of casein kinase 1 and functional analysis of new doubletime mutants in Frontiers in Physiology 13 : article number: 1062632.
DOI: 10.3389/fphys.2022.1062632
Kozelková T., Doležel D., Grunclová L., Kučera M., Perner J., Kopáček P. (2021) Functional characterization of the insulin signaling pathway in the hard tick Ixodes ricinus Ticks and Tick-borne Diseases 12 : 101694.
DOI: 10.1016/j.ttbdis.2021.101694
Netušil R., Tomanová K., Chodáková L., Chvalová D., Doležel D., Ritz T., Vacha M. (2021) Cryptochrome-dependent magnetoreception in a heteropteran insect continues even after 24 h in darkness. Journal of Experimental Biology 224 : article number: jeb243000.
DOI: 10.1242/jeb.243000
Kaniewska M., Vaněčková H., Doležel D., Kotwica-Rolinska J. (2020) Light and temperature synchronizes locomotor activity in the linden bug, Pyrrhocoris apterus. Frontiers in Physiology 11 : article number: 242.
DOI: 10.3389/fphys.2020.00242
Kotwica-Rolinska J., Krištofová L., Chvalová D., Pauchová L., Provazník J., Hejníková M., Sehadová H., Lichý M., Vaněčková H., Doležel D. (2020) Functional analysis and localisation of a thyrotropin-releasing hormone-type neuropeptide (EFLa) in hemipteran insects. Insect Biochemistry and Molecular Biology 122 : article number: 103376.
DOI: 10.1016/j.ibmb.2020.103376
Smýkal V., Pivarči M., Provazník J., Bazalová O., Jedlička P., Lukšan O., Horák A., Vaněčková H., Beneš V., Fiala I., Hanus R., Doležel D. (2020) Complex evolution of insect insulin receptors and homologous decoy receptors, and functional significance of their multiplicity. Molecular Biology and Evolution 37 : 1775-1789.
DOI: 10.1093/molbev/msaa048
Bartoš P., Netušil R., Slabý P., Doležel D., Ritz T., Vácha M. (2019) Weak radiofrequency fields affect the insect circadian clock. Journal of the Royal Society Interface 16 : article number: 20190285.
DOI: 10.1098/rsif.2019.0285
Doležel D. (2019) Chronobiology: The circadian clock under extreme photoperiods. Current Biology 29 : R1176-R1178.
DOI: 10.1016/j.cub.2019.09.061
Kotwica-Rolinska J., Chodáková L., Chvalová D., Krištofová L., Fenclová I., Provazník J., Wu C., Bertolutti M., Doležel D. (2019) CRISPR/Cas9 genome editing introduction and optimization in the non-model insect Pyrrhocoris apterus. Frontiers in Physiology 10 : article 891.
DOI: 10.3389/fphys.2019.00891

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Biology Centre CAS
Institute of Entomology
Branišovská 1160/31
370 05 České Budějovice

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