| Total ESTs |
|
Total number of ESTs associated with promoter sequence by high stringency BLAST.
Expect value used for Blast was e-21, additional filtering practically removed matches of expect > e-30.
|
| Positive 5-prime ends |
|
| Number of 5' ESTs ends mapping in the region +30 to - 5 relative to transcription start point. |
sample 1
sample 2
sample 3
|
| Overshooting 5-prime ends |
|
| Number of longer 5' ESTs ends. |
sample 1
sample 2
sample 3
sample 4
|
| Minimal positive : overshooting ratio |
|
Ratio of 5' ESTs ends mapping in the region +30 to -20 versus longer 5' ESTs ends. Low values sugest that
the downstream promoter is very weak or the transcription start poin is missmapped.
|
| Minimal upstream sequence lenght |
|
Retrieve upstream promoter sequences of certain minimal length. The sequences range in size from 60 to 1000 bp, average lenght is near 750 bp
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| Minimal downstream sequence lenght |
|
The immediatelly downstream transcribed sequence was used as query for the high stringency Blast search.
|
| Promoter pos. |
|
Minus one nucleotide placement in the parent sequence. This is the most 3' nucleotide in the PRESTA upstream sequence.
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| Expressed in |
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Looks for the occurence of certain words in the tissue or organ source of Ests coupled to the particular promoter.
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| Upstream seq. |
|
Maximum number of bases to report from the upstream sequence. The 5' end of the reported sequence is located upstream, the 3' end maps to nucleotide -1.
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| Downstream seq. |
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Maximum number of bases to report from the downstream sequence. The 5' end of the reported sequence is located at nucleotide +1.
|