Mgr. David Doležel, Ph.D.

Job Position: Head of Laboratory - Laboratory of Molecular Chronobiology

Contact details

Phone: 420387775239
E-mail: dolezel@entu.cas.cz

Description

MAIN RESEARCH INTERESTS

  • Insect seasonality – diapause; architecture of photoperiodic timer (at molecular, genetic and cellular levels), geographic variability of the photoperiodic timer, Juvenile hormone signaling in reproduction of insects.
  • Pyrrhocoris apterus as a new insect model – endocrinology (neuropeptides, evolution of neuropeptide receptors), reproductive behavior, circadian clock, phylogeography of P. apterus and its adaptation, technique development (RNA interference, genome editing, organ cultures).
  • Drosophila melanogaster – temperature compensation of circadian clock, CRISPR/CAS9 as a tool for targeted mutagenesis.
  • Evolution of circadian genes – magnetoreception (using insect models), circadian clock in protozoa (small hobby project on Chromera velia, ancestor of Plasmodium).
For details check current research projects on the web page of the Laboratory of Molecular Chronobiology

 

MAJOR COLLABORATIONS

  • Roger Butlin, University of Sheffield, UK; Project: Phylogeography of P. apterus,
  • Christian Wegener, University of Wuerzburg, Germany; Project: Neuropeptides in P. apterus
  • Vladimir Benes, GeneCore, EMBL, Heildeberg, Germany; Project: P. apterus genome & transcriptome
  • Richard Pokorny, Philipps-University, Marburg, Germany; Project: structure & biochemistry of insect Cryptochrome proteins
  • Fernando Noriega, Florida Int. University, USA; Project: Juvenile hormone in P. apterus
  • Robert Hanus, Institute of Organic Chemistry and Biochemistry, CAS, Czech Republic; Project: Juvenile hormone in termite cast differentiation
  • Christopher Wheat, Stockholm U., Sweden – Population genomics in P. apterus – Pool-seq data analysis

 

 

Mentor of PhD students

Current:

Past:

 

Mentor of undergraduate students

Current:

Past:

  • Marion Sieber (MSc – 2014),
  • Katka Švehlová (MSc – 2013)
  • Olga Pecková (MSc – 2012)
  • Martina Jonáková (MSc – 2012)
  • Silvie Fexová (MSc – 2010)
  • Olga Bazalová (MSc – 2010)
  • Juraj Dobrovodský (BSc - 2012 )
  • Olga Pecková (BSc 2010)
  • Eva Stehliková (BSc 2010)

 

 

Curriculum vitae

BORN: 1974; Czech Republic

CURRENT POSITION(S)

group leader, Institute of Entomology, Biology Center CAS, Czech R. (full appointment)

lecturer, Faculty of Science, University of South Bohemia (current appointment 40%)

 

EDUCATION AND RESEARCH EXPERIENCE

1994-1997   B.Sc. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

1997             three-month-stay, The University of Arizona (Tucson), David Galbraith´s lab., USA.

1997-1999   M.Sc. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

1999-2003   Ph.D. studies, Faculty of Biological Sciences, U. of South Bohemia, Č. Budějovice, CZ

2004-2007   Postdoctoral Fellow, Brandeis U. (Laboratory of J. C. Hall), Waltham, MA, USA.

2007-2010   Postdoctoral Fellow, Institute of Entomology, Biology Center ASCR, Č. Budějovice, CZ

2011-2013   Researcher, Institute of Entomology, Biology Center ASCR, Č. Budějovice, CZ

Since 2013   Group leader, Laboratory of Chronobiology, Biology Center ASCR, Č. Budějovice, CZ

 

TEACHING

Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

  • Advanced Methods in Molecular Biology (KMB/603) - complete course, 2011- present
  • Methods in Functional Genomics (KMB/217)
  • Methods of Molecular Biology (KMB/770)
  • Cell Regulation and Signaling (KMB/614) – two lectures (Chronobiology), 2009-present
  • Modern methods of Chronobiology 2009-2015
  • Methods in Molecular Biology: 2009-2011
  • 1 Ph.D., 6 M.Sc., 3 B.Sc. defended under supervision of David Dolezel

 

SUPERVISION OF GRADUATE STUDENTS

  • Adam Bajgar, defended Ph.D. in 2013

Currently supervisor of 7 Ph.D. students:

  • Olga Bazalova, topic: The temperature in insect circadian clocks, Ph.D. defence scheduled for October 2016
  • Jan Provaznik, topic: Genomics and transcriptomics of Pyrrhocoris apterus,
  • Lenka Chodakova, topic: Molecular architecture of photoperiodic clock in the Linden bug, P. apterus
  • Martin Pivarci, topic: Phylogeography of the Linden bug, Pyrrhocoris apterus
  • Marketa Hejnikova, topic: Neurohormones and their genes in the retrocerebral complex of P. apterus
  • Bulah Chia-hsiang Wu, topic: Geographic variability in circadian and photoperiodic clocks
  • Samarjeet Singh, topic: Functional analysis of circadian genes in Drosophila
  • External mentor for Enrico Bertolini (University of Wuerzburg, Germany) under INsecTIME project, http://insectime.org/main/, topic: Clock networks in insects

 

SUPERVISION OF POSTDOCTORAL FELLOWS

  • Joanna Kotwica-Rolinska, September 2013 – June 2015, research: Neuropeptide inventory in P. apterus,  evolution of insulin receptors, genome editing of P. apterus

 

WORKSHOPS ORGANIZED

  • Methods of Functional Genomics, Ceske Budejovice, Czech Rep.: March 2013; April 20-22, 2015, Series of lectures and practicals covering genomics, proteomics, imaging & cell-based screen, taught by experts from EMBL core facilities
  • Confocal microscopy workshop, Ceske Budejovice, Czech Rep.: August 5-9, 2013; July 27-31, 2015, lectures and hands on training (basics, FRET, FRAP, image analysis software)
  • Workshop on molecular dynamics and PELE, lecturer: Victor Guallar (Barcelonona Supercomputing Center), Ceske Budejovice, Czech Rep.: June 9-14, 2013

 

FUNDING

  • Grant Agency of the Academy of the Czech Republic - Protein Protein Interaction (2008 - 2012; PI)
  • National Science Foundation - Molecular Mechanism of Insect Diapause (2008 - 2010; PI)
  • National Science Foundation - Evolution of Circadian Clocks in Insects (2014 - 2016; PI)
  • Ministry of Education of CR - Juvenile Hormone in Pyrrhocoris apterus (2014 - 2016; PI)
  • National Science Foundation - Neuroendocrine Control of Insect Reproductive Diapause (2015 - 2017; co-PI)
  • ModBiolin Project (FP7/2007e2013, grant No. 316304), leader of WP6: Acquisition of Instrument for infrastructure development (budget of WP6 463, 400 €)
  • Project Molecularization, joint effort of Faculty of Sciences & Biology center; head for Biology Center part, organizing workshops & practical training courses
  • Principal investigator on 2 Grants of Ministry of Education of the CR aiming at Support of ~30 PhD student at the department of molecular biology (budget 310, 000 €)

 

INVITED LECTURES AND CONFERENCE PRESENTATIONS

  • Marie Curie ITN, Jyväskyla, Finland, May 2010: Circadian and Photoperiodic Clock
  • Marie Curie ITN, Jyväskyla, Finland, May 2010: Molecular tools applied to non-model insects
  • Institute of Molecular Life Sciences, University of Zurich, April 2012
  • University of Jyväskyla, Jyväskyla, Finland, November 2012
  • MPI for Chemical Ecology, Jena, Germany, November 2012
  • Institute of Physiology, ASCR, Prague, February 2013
  • Institute of Organic Chemistry and Biochemistry, ASCR, Prague, April 2013
  • Wuerzburg University, Germany, October 2013
  • University of Leicester, UK, December 2013
  • 7th International Symposium on Molecular Insect Science, Amsterdam, July 2014
  • Wageningen, Netherlands, July, 2014
  • Osaka, Japan, November 2014
  • Kobe, Japan, November 2014
  • Tsukuba, Japan, November 2014
  • NTU, Taiapei, Taiwan, November 2014
  • University of Sheffield, UK, December 2014
  • Swansea University, Wales, UK, March 24, 2015
  • Institute of Organic Chemistry and Biochemistry, ASCR, Prague, October 2015
  • University of Krakow, Poland, March 2016
  • Stellenbosch University, Republic of South Africa, April, 2016

 

PLANNED CONFERENCE PERESENTATIONS

  • Time and Light, Viena, Austria, May 2016 (poster: Circadian clock in protist Chromera velia)
  • SRBR meeting, Florida, USA, May 2016 (talk: Circadian genes, Photoperiodic clock and Diapause in insect, Pyrrhocoris apterus)
  • International Congress of Entomology, Orlando, USA, September 2016 (talk in symposium: Neuro-Endocrine Regulation of Reproduction in Insects)

 

MEMBERSHIP IN SCIENTIFIC SOCIETIES

  • The Society on Research on Biological Rhythms,
  • network member, Marie  Curie ITN “INsecTIME” http://insectime.org/main/ (FP7)
  • International Society for Evolutionary Protistology – 1997-2001

 

OTHER PROFESSIONAL ACTIVITIES

Reviewer for journals (on regular basis): Insect Biochemistry and Molecular Biology, Proceedings of the National Academy of Sciences USA, Journal of Biological Rhythms, Journal of Insect Physiology, PLoS ONE, Entomological Science

Referee of a Ph.D. thesis: (University of Jyvaskyla, Finland; University of Leicester, UK; Charles University, Prague)

 

PUBLICATIONS

26 peer-reviwed papers (including 1x invited review), >500 citations,  h-index 14 (Scopus), 13 (WOS)

 

 

Publications under review:

Pivarciova, Provaznik, Vaneckova, Pivarci, Peckova, Bazalova, Wu, Cada, Kment, Kotwica-Rolinska, Dolezel: Unexpected geographic variability of free running period in the linden bug, Pyrrhocoris apterus (in revision)

 

Publications
Total found: 33 records
Bazalová O., Doležel D. (2017) Daily Activity of the Housefly, Musca domestica, Is Influenced by Temperature Independent of 3' UTR period Gene Splicing G3: Genes, Genomes, Genetics 7 : 2637-2649.
DOI: 10.1534/g3.117.042374
Ibrahim E. A. S., Hejníková M., Shaik H. A., Doležel D., Kodrík D. (2017) Adipokinetic hormone activities in insect body infected by entomopathogenic nematode. J. Insect Physiol. 98 : 347-355.
DOI: 10.1016/j.jinsphys.2017.02.009
Kotwica-Rolinska J., Pivarčiová L., Vaněčková H., Doležel D. (2017) The role of circadian clock genes in the photoperiodic timer of the linden bug, Pyrrhocoris apterus, during the nymphal stage. Physiological Entomology 42 : 266-273.
DOI: 10.1111/phen.12197
Bazalová O., Kvicalova M., Valkova T., Slaby P., Bartos P., Netusil R., Tomanova K., Braeunig P., Lee H., Šauman I., Damulewicz M., Provazník J., Pokorny R., Doležel D., Vacha M. (2016) Cryptochrome 2 mediates directional magnetoreception in cockroaches. Proceedings of the National Academy of Sciences of the United States of America 113 : 1660-1665.
DOI: 10.1073/pnas.1518622113
Pivarčiová L., Vaněčková H., Provazník J., Wu C., Pivarči M., Pecková O., Bazalová O., Čada Š., Kment P., Kotwica-Rolinska J., Doležel D. (2016) Unexpected geographic variability of the free running period in the linden bug, Pyrrhocoris apterus. Journal of Biological Rhythms 31 : 568-576.
DOI: 10.1177/0748730416671213
Urbanová V., Bazalová O., Vaněčková H., Doležel D. (2016) Photoperiod regulates growth of male accessory glands through juvenile hormone signaling in the linden bug, Pyrrhocoris apterus. Insect Biochemistry and Molecular Biology 70 : 184-190.
Závodská R., Kobelková A., Šauman I., Bazalová O., Doležel D. (2016) Exprese genů biologických hodin v mozku zavíječe moučného (Ephestia kuehniella). Sborník abstraktů z konference 92. Fyziologické dny - konference české a slovenské fyziologické společnosti, 2.-4. února 2016, Přírodovědecká fakulta JU, České Budějovice, pp. 104, ISBN 978-80-7394-563-3
Doležel D. (2015) Photoperiodic time measurement in insects. Current Opinion in Insect Science 7 : 98-103.
DOI: 10.1016/j.cois.2014.12.002
Kobelková A., Závodská R., Šauman I., Bazalová O., Doležel D. (2015) Expression of clock genes /period/ and /timeless /in the central nervous system of the Mediterranean flour moth, Ephestia kuehniella Journal of Biological Rhythms 30 : 104-116.
DOI: 10.1177/0748730414568430
Poupardin R., Schöttner K., Korbelová J., Provazník J., Doležel D., Pavlinic D., Beneš V., Košťál V. (2015) Early transcriptional events linked to induction of diapause revealed by RNAseq in larvae of drosophilid fly, Chymomyza costata. BMC Genomics 16 : 702.
DOI: 10.1186/s12864-015-1907-4
Smýkal V., Bajgar A., Provazník J., Fexová S., Buřičová M., Takaki K., Hodková M., Jindra M., Doležel D. (2014) Juvenile hormone signaling during reproduction and development of the linden bug, Pyrrhocoris apterus. Insect Biochemistry and Molecular Biology 45 : 69-76.
DOI: 10.1016/j.ibmb.2013.12.003
Bajgar A., Doležel D., Hodková M. (2013) Endocrine regulation of non-circadian behavior of circadian genes in insect gut. Journal of Insect Physiology 59 : 881-886.
DOI: 10.1016/j.jinsphys.2013.06.004
Bajgar A., Jindra M., Doležel D. (2013) Autonomous regulation of the insect gut by circadian genes acting downstream of juvenile hormone signaling. Proceedings of the National Academy of Sciences of the United States of America 110 : 4416-21.
DOI: 10.1073/pnas.1217060110
Závodská R., Fexová S., Von Wowern G., Han G., Doležel D., Šauman I. (2012) Is the Sex Communication of Two Pyralid Moths, Plodia interpunctella and Ephestia kuehniella, under Circadian Clock Regulation? Journal of Biological Rhythms 27 : 206-216.
DOI: 10.1177/0748730412440689
Kobelková A., Bajgar A., Doležel D. (2010) Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata Journal of Biological Rhythms 25 : 399-409.
DOI: 10.1177/0748730410385283

Documents to download:
Kobelkova 2010 (pdf)
Doležel D., Zdechovanová L., Šauman I., Hodková M. (2008) Endocrine-dependent expression of circadian clock genes in insects. Cell. Mol. Life Sci. 65 : 964-969.
DOI: 10.1007/s00018-008-7506-7
Košťál V., Doležel D., Tollarová M. (2008) Dynamism in physiology and gene transcription during reproductive diapause in a heteropteran bug, Pyrrhocoris apterus. J. Insect Physiol. 54 : 77-88.
Codd V., Doležel D., Stehlík J., Piccin A., Garner K., Seth R., Straatman K., Louis E., Costa R., Šauman I., Kyriacou C., Rosato E. (2007) Circadian rhythm gene regulation in the housefly, Musca domestica. Genetics 177 : 1539-1551.
DOI: 10.1534/genetics.107.079160

Documents to download:
2007-Genetics (pdf)
Doležel D., Šauman I., Košťál V., Hodková M. (2007) Photoperiodic and food signals control expression pattern of the clock gene, period, in the linden bug, Pyrrhocoris apterus. J. Biol. Rhythms 22 : 335-342.
DOI: 10.1177/0748730407303624
Doleželová E., Doležel D., Hall J. (2007) Rhythm defects caused by newly engineered null mutations in Drosophila’s cryptochrome gene. Genetics 177 : 329-345.
DOI: 10.1534/genetics.107.076513

Documents to download:
Dolezelova et al. (2007) (pdf)

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CONTACT

Biology Centre CAS
Institute of Entomology
Branišovská 1160/31
370 05 České Budějovice

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