Researcher, Laboratory of Analytical Biochemistry
Sample preparation techniques
Derivatization with alkyl chloroformates
GC-FID, GC-MS analysis
Šimek P., Zahradníčková H., Cimlová J., Makuderová L., Hušek P. (2012) Alkyl chloroformate-mediated derivatization – extraction platform for in-situ metabolite labeling and parallel nonchiral and chiral GC-MS & LC-MS analysis In: Book of Abstracts and Program from Advances in Chromatography and Electrophoresis & Chiranal 2012, June 11-14, 2012, Olomouc, pp. 46-47. (Acta Universitatis Palackianae Olomucensis).
Hušek P. (2012) Letter to the Editor regarding “GC–MS with ethyl chloroformate derivatization for comprehensive analysis of metabolites in serum and its application to human uremia". Anal. Bioanal. Chem. 403: 2785-2786.
Hušek P., Švagera Z., Hanzlíková D., Šimek P. (2012) Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography. J. Pharm. Biomed. Anal. 67- 68: 159-162.
Švagera Z., Hanzlíková D., Šimek P., Hušek P. (2012) Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography–mass spectrometry Anal. Bioanal. Chem. 402: 2953-2963.
Cimlová J., Kružberská P., Švagera Z., Hušek P., Šimek P. (2012) In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry J. Mass. Spectrom. 47: 294-302.
